Molecular cloning and biochemical characterization of a lipoxygenase in almond (Prunus dulcis) seed
نویسندگان
چکیده
منابع مشابه
Cloning and characterization of profilin (Pru du 4), a cross-reactive almond (Prunus dulcis) allergen.
BACKGROUND The identity of allergenic almond proteins is incomplete. OBJECTIVE Our objective was to characterize patient IgE reactivity to a recombinant and corresponding native almond allergen. METHODS An almond cDNA library was screened with sera from patients with allergy for IgE binding proteins. Two reactive clones were sequenced, and 1 was expressed. The expressed recombinant allergen...
متن کاملcdna cloning, expression and characterization of an allergenic 60s ribosomal protein of almond (prunus dulcis)
tree nuts, including almond (prunus dulcis) are a source of food allergens often associated with life-threatening allergic reactions in susceptible individuals. although the proteins in almonds have been biochemically characterized, relatively little has been reported regarding the identity of the allergens involved in almond sensitivity. the present study was undertaken to identify the allerge...
متن کاملAnatomy and cell wall polysaccharides of almond (Prunus dulcis D. A. Webb) seeds.
The anatomy of Prunus dulcis was analyzed by applying several differential staining techniques and light microscopy. Prunus dulcis seed has a thin and structurally complex seed coat, with lignified cellulosic tissue. The embryo has two voluminous cotyledons. Cotyledon cells have a high number of protein and lipid bodies, some of which have phytin. The provascular tissue, located in the cotyledo...
متن کاملIdentification of stylar RNases associated with gametophytic self-incompatibility in almond (Prunus dulcis).
Stylar proteins of 13 almond (Prunus dulcis) cultivars with known S-genotypes were surveyed by IEF and 2D-PAGE combined with immunoblot and N-terminal amino acid sequence analyses to identify S-RNases associated with gametophytic self-incompatibility (SI) in this plant species. RNase activities corresponding to Sa and Sb, two of the four S-alleles tested, were identified by IEF and RNase activi...
متن کاملThe Small-RNA Profiles of Almond (Prunus dulcis Mill.) Reproductive Tissues in Response to Cold Stress
Spring frost is an important environmental stress that threatens the production of Prunus trees. However, little information is available regarding molecular response of these plants to the frost stress. Using high throughput sequencing, this study was conducted to identify differentially expressed miRNAs, both the conserved and the non-conserved ones, in the reproductive tissues of almond tole...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2001
ISSN: 0014-2956
DOI: 10.1046/j.1432-1327.2001.02020.x